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The tissue was ground in 2 ml Eppendorf tubes containing two 4 mm glass beads using a Retsch ® M300 TissueLyser (Retsch , Haan, Germany) at 30 Hz for several minutes. ABA was extracted by adding 500 μl cold 80% MeOH to each sample and samples were mixed on an overhead shaker in the dark overnight.
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The aim of this study was to examine the effect of abscisic acid (ABA), sucrose, and auxin on grape fruit development and to assess the mechanism of these three factors on the grape fruit ripening process. Different concentrations of ABA, …
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All other plants were grown using the same conditions used for the CHX assays. Media were discarded and 1 ml of fresh media with either 50 μ m MG132 [final 0.5% (v/v) DMSO] or with 0.5% (v/v) DMSO was added. For ABA treatment, media were discarded …
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Elution 1 (ABA and IAA) was reconstituted in 1 mL of the initial mobile phase conditions (0.08% CH 3 COOH:CH 3 OH; 95:5 v/v), and a 20 μL aliquot was injected onto a Kinetex C18 column (2.1 × 100 mm, 1.9 μm solid core, 0.35 μm porous shell; Phenomenex, Torrance, CA, U.S.A.) for HPLC-ESI(−)-MS/MS. ABA and IAA were eluted with an increasing ...
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Plants are subjected to a variety of environmental stressors, including salinity, drought and pathogens, and have consequently developed a sophisticated sensing system. In Arabidopsis, malectin-like LRR-RLK impaired oomycete susceptibility 1 (IOS1, AT1G51800) plays an important role in both abiotic and biotic stresses by attenuating abscisic acid responses and regulating pattern-triggered ...
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